蒿甲醚治疗免疫抑制大鼠卡氏肺孢子虫的实验性试验。
An experimental trial of artemether in treatment of Pneumocystis carinii in immunosuppressed rats.
作者信息
Chen Y T, Ma L, Mei Q, Tang Y, Liao X G
机构信息
Institute of Infectious and Parasitic Diseases, Chongqing University of Medical Sciences.
出版信息
Chin Med J (Engl). 1994 Sep;107(9):673-7.
An immunosuppressed rat model was established by injecting cortisone acetate 25 mg/rat twice a week for 4 weeks and 12.5 mg/rat for another 2 weeks subcutaneously. A development of Pneumocystis carinii pneumonia (PCP) was found at the end of the 6th week in all rats. These rats were injected intramuscularly with artemether at 100 mg/kg once a day for 5 consecutive days. All rats were necropsied at the end of the 8th week. The lung impression smears were stained with Gomori's stain, and Pneumocystis carinii cysts were counted. The ultrastructural changes of trophozoite, precyst and cyst were investigated by transmission electron microscopy on the 7th day after treatment.
通过每周两次皮下注射醋酸可的松25mg/只，持续4周，之后每周两次皮下注射12.5mg/只，持续2周，建立免疫抑制大鼠模型。在第6周结束时，所有大鼠均出现卡氏肺孢子虫肺炎（PCP）。这些大鼠每天一次肌肉注射蒿甲醚100mg/kg，连续注射5天。所有大鼠在第8周结束时进行尸检。肺印片涂片用戈莫里氏染色法染色，并对卡氏肺孢子虫囊肿进行计数。在治疗后第7天，通过透射电子显微镜研究滋养体、包囊前期和包囊的超微结构变化。
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