Luo L Q, Zhang Y H
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1994 Jul;16(4):251-4.
gamma-Interferon (IFN-gamma) was detected by ELISA assay in ascitic fluid from a number of ovarian cancer patients. To study its clinical significance, the effect of IFN-gamma on the metastatic potential of a mouse mammary adenocarcinoma, MA-891, was explored. Pretreatment of the tumor cells in vitro for 48hr with recombinant INF-gamma significantly increased the number of lung tumor nodules after i. v. or s. c. inoculation into (TA2 x 615) F1 mice. In contrast, when recombinant IFN-alpha pretreated MA-891 cells were likewise injected into mice significant decrease in metastatic potential was seen. The study in vitro indicated that pretreatment of the tumor cells with IFN-gamma but not with IFN alpha resulted in a decrease in susceptibility to NK cell cytotoxicity. In as much as both IFN-alpha and IFN-gamma can induce MHC class I expression on target cells. The increase in metastatic potential of IFN-gamma-treated tumor cells can be explained only partially on the basis of their reduced NK cells susceptibility.
通过酶联免疫吸附测定法（ELISA）在许多卵巢癌患者的腹水中检测到γ-干扰素（IFN-γ）。为了研究其临床意义，探讨了IFN-γ对小鼠乳腺腺癌MA-891转移潜能的影响。用重组IFN-γ在体外对肿瘤细胞预处理48小时后，经静脉或皮下接种到（TA2×615）F1小鼠体内，肺肿瘤结节的数量显著增加。相比之下，当将经重组IFN-α预处理的MA-891细胞同样注射到小鼠体内时，转移潜能显著降低。体外研究表明，用IFN-γ而非IFN-α预处理肿瘤细胞会导致对自然杀伤（NK）细胞细胞毒性的敏感性降低。由于IFN-α和IFN-γ都能诱导靶细胞上主要组织相容性复合体（MHC）I类分子的表达，IFN-γ处理的肿瘤细胞转移潜能的增加只能部分基于其对NK细胞敏感性的降低来解释。
