[抗人肝癌单克隆抗体轻链可变区基因序列]
[Sequence of light chain variable region gene of a monoclonal antibody to human hepatocarcinoma].
作者信息
Yang P, Liu Y F, Hu C M
机构信息
Department of Pathology, Fourth Military Medical University, Xian.
出版信息
Zhonghua Zhong Liu Za Zhi. 1994 Jul;16(4):263-5.
The isolation of the rearranged immunoglobulin genes from a hybridoma cell line, which is a prerequisite for the construction of a recombinant antibody, can easily be achieved by PCR. We have determined the nucleotide sequence of the variable region of L chain of a high affinity monoclonal antibody HAb27 directed to human hepatocarcinoma. Analysis of the nucleotide sequence of the light chain V region of HAb27 revealed that the V kappa sequence was 85% identical to germline V kappa Ox1 sequence.
从杂交瘤细胞系中分离重排的免疫球蛋白基因是构建重组抗体的前提条件，通过聚合酶链反应（PCR）很容易实现。我们已经测定了针对人肝癌的高亲和力单克隆抗体HAb27轻链可变区的核苷酸序列。对HAb27轻链V区核苷酸序列的分析表明，Vκ序列与种系VκOx1序列的同源性为85%。
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