Kimata H, Lindley I
Department of Pediatrics, Kyoto University Hospital, Japan.
Eur J Immunol. 1994 Dec;24(12):3237-40. doi: 10.1002/eji.1830241250.
The effect of interleukin-8 (IL)-8 on human B cell growth, as determined by thymidine uptake and viable cell numbers was studied. IL-8 inhibited IL-4-induced growth of B cells costimulated with anti-mu antibodies (Ab) or Staphylococcus aureus Cowan strain I (SAC) in a dose-dependent fashion. In contrast, IL-8 did not inhibit IL-2-induced growth of B cells. The IL-8-mediated inhibition was specific, since it was blocked by anti-IL-8 mAb but not by control IgG1. Moreover, anti-tumor necrosis factor-alpha (anti-TNF-alpha) Ab blocked IL-8-mediated inhibition. On the other hand, TNF-alpha, but not other cytokines including IL-1 beta, IL-3, IL-5, IL-6, interferon-alpha (IFN-alpha) or IFN-gamma, inhibited IL-4-mediated growth, and inhibition by TNF-alpha was blocked by anti-TNF-alpha Ab but not by control IgG. IL-4 had no effect on TNF-alpha binding by B cells while it decreased TNF-alpha production by B cells. IL-8 had no effect in binding of IL-4, IL-2 or TNF-alpha by B cells, however, it enhanced TNF-alpha production by B cells. These results indicate that IL-8 inhibited IL-4-induced human B cell growth by enhancement of endogenous TNF-alpha production.
通过胸苷摄取和活细胞数量测定了白细胞介素-8（IL-8）对人B细胞生长的影响。IL-8以剂量依赖的方式抑制抗μ抗体（Ab）或金黄色葡萄球菌考恩I株（SAC）共刺激的IL-4诱导的B细胞生长。相反，IL-8不抑制IL-2诱导的B细胞生长。IL-8介导的抑制是特异性的，因为它被抗IL-8单克隆抗体阻断，而不是被对照IgG1阻断。此外，抗肿瘤坏死因子-α（抗TNF-α）抗体阻断IL-8介导的抑制。另一方面，TNF-α而非包括IL-1β、IL-3、IL-5、IL-6、干扰素-α（IFN-α）或IFN-γ在内的其他细胞因子抑制IL-4介导的生长，并且TNF-α的抑制被抗TNF-α抗体阻断，而不是被对照IgG阻断。IL-4对B细胞结合TNF-α没有影响，而它降低了B细胞产生TNF-α。IL-8对B细胞结合IL-4、IL-2或TNF-α没有影响，然而，它增强了B细胞产生TNF-α。这些结果表明，IL-8通过增强内源性TNF-α的产生来抑制IL-4诱导的人B细胞生长。
