[采用放射性碘标记大鼠血清白蛋白外推法测定Wistar-今市品系雌性无特定病原体大鼠的血容量]

[Blood volume determination in female SPF rats of Wistar--Imamichi strain by an extrapolation method with radioiodinated rat serum albumin].

作者信息

Ishikawa J, Hayashi T

机构信息

Central Research Laboratory, National Defense Medical College, Saitama, Japan.

出版信息

Jikken Dobutsu. 1994 Oct;43(4):535-9. doi: 10.1538/expanim1978.43.4_535.

DOI:10.1538/expanim1978.43.4_535

PMID:7805797

Abstract

The total blood volume of SPF female rats of Wistar-Imamichi strain, a strain that shows a 4-day estrous cycle at very high rates and is widely used in the study of reproductive biology in Japan, was estimated from blood radioactivity after the intravenous administration of 125I-rat serum albumin. The radioactivity of blood showed a rapid decrease until 10 min after injection and a slow decay thereafter. Radioactivity at time zero was estimated by extrapolation from this slow decay curve, and the blood volume was calculated. From 30 rats weighing 150-300 g we obtained the following equation between blood volume (BLV) and body weight (BW), and a high correlation coefficient (r): BLV = 8,4007 + 0.0430 x BW, r2 = 0.92. The blood volume per body weight decreased linearly with increasing body weight, and we obtained the following equation: BLD/100 g BW = 12.25-0.0174 x BW at r2 = 0.92.

摘要

Wistar-Imamichi品系的无特定病原体（SPF）雌性大鼠在日本广泛用于生殖生物学研究，该品系具有很高比例的4天发情周期。通过静脉注射125I-大鼠血清白蛋白后血液放射性来估算其总血容量。血液放射性在注射后10分钟内迅速下降，此后缓慢衰减。通过从该缓慢衰减曲线外推来估算零时的放射性，并计算血容量。从30只体重为150 - 300克的大鼠中，我们得出了血容量（BLV）与体重（BW）之间的以下方程，以及较高的相关系数（r）：BLV = 84007 + 0.0430×BW，r2 = 0.92。每体重的血容量随体重增加呈线性下降，我们得出了以下方程：每100克体重的血容量（BLD/100 g BW）= 12.25 - 0.0174×BW，r2 = 0.92。