Martin S K, Carroll R, Benig M, Steiner D F
Department of Biochemistry, University of Chicago, IL 60637.
FEBS Lett. 1994 Dec 19;356(2-3):279-82. doi: 10.1016/0014-5793(94)01284-9.
The prohormone convertases PC2 and PC3 have been shown to catalyze the processing of proinsulin to insulin in pancreatic beta-cells. In these studies we have compared the effects of glucose on PC2 and PC3 biosynthesis in freshly isolated islets from normal and hyperglycemic (ob/ob) mice. In contrast to normal islets [Alarcón, et al. (1993) J. Biol. Chem. 268, 4276] the biosynthesis of both PC2 and PC3 is stimulated by glucose, parallel to the stimulation of proinsulin in the (ob/ob) islets. Inhibition of PC2 biosynthesis by glucose in normal islet non beta-cells may obscure stimulation of PC2 biosynthesis in normal islet beta-cells.
激素原转化酶PC2和PC3已被证明可催化胰岛β细胞中胰岛素原加工生成胰岛素。在这些研究中，我们比较了葡萄糖对正常小鼠和高血糖（ob/ob）小鼠新鲜分离胰岛中PC2和PC3生物合成的影响。与正常胰岛不同[阿拉孔等人（1993年）《生物化学杂志》268卷，4276页]，PC2和PC3的生物合成在（ob/ob）胰岛中均受到葡萄糖刺激，这与胰岛素原的刺激情况相似。正常胰岛非β细胞中葡萄糖对PC2生物合成的抑制作用可能掩盖了正常胰岛β细胞中PC2生物合成所受到的刺激。
