铜绿假单胞菌细胞毒素的细胞溶解作用机制：细胞毒素在靶膜上的寡聚化。

Mechanism of the cytolytic action of Pseudomonas aeruginosa cytotoxin: oligomerization of the cytotoxin on target membranes.

作者信息

Ohnishi M, Hayashi T, Tomita T, Terawaki Y

机构信息

Department of Bacteriology, Shinsyu University School of Medicine, Matsumoto, Japan.

出版信息

FEBS Lett. 1994 Dec 19;356(2-3):357-60. doi: 10.1016/0014-5793(94)01311-x.

DOI:10.1016/0014-5793(94)01311-x

PMID:7805872

Abstract

Pseudomonas aeruginosa cytotoxin (CTX) is thought to be a pore-forming polypeptide of 29 kDa. To study whether CTX assembles into oligomer on target membranes, we solubilized membrane-bound toxin with 1% sodium dodecyl sulfate (SDS) at 25 degrees C and analyzed its molecular size using SDS-polyacrylamide gel electrophoresis and immunoblot analysis. The results indicate that CTX forms a complex of approximately 145 kDa on the surface of erythrocytes and lipid vesicles, and that the complex formation is closely correlated with the toxin-induced permeabilization of target membranes. Thus, CTX may assemble into a pore-forming oligomer on target membranes.

摘要

铜绿假单胞菌细胞毒素（CTX）被认为是一种29 kDa的成孔多肽。为了研究CTX是否在靶膜上组装成寡聚体，我们在25℃下用1%十二烷基硫酸钠（SDS）溶解膜结合毒素，并使用SDS-聚丙烯酰胺凝胶电泳和免疫印迹分析来分析其分子大小。结果表明，CTX在红细胞和脂质体表面形成了一个约145 kDa的复合物，并且复合物的形成与毒素诱导的靶膜通透性密切相关。因此，CTX可能在靶膜上组装成一个成孔寡聚体。