Roncaroli F, Dina R, Geuna M, Reato G, Ponti R, Palestro G
Institute of Pathology, University of Bologna, Italy.
Haematologica. 1994 Jul-Aug;79(4):322-7.
In the last few years many studies have been published on GM-CSF receptors, focusing on molecular structure, function and distribution. Nevertheless, protocols for detecting GM-CSF receptors on formalin-fixed paraffin-embedded histological sections, to our knowledge, have not been described.
A method based on non-isotopic in situ hybridization (ISH) using a 21-base antisense DNA oligoprobe whose 3'-end was labeled with digoxigenin 11-dUTP was devised. The probe was applied on 20 routinely processed bone marrow trephine biopsies which were considered as positive controls.
The hybridization signal was seen in myeloid cells, erythroid progenitors and rare megakaryocytes.
Non-isotopic ISH represents an alternative to current methodologies for the assessment of GM-CSF receptor expression; since it is suitable for routinely processed samples, it can be regarded as a helpful tool for diagnostic determination of GM-CSF receptors in tumors from patients receiving GM-CSF and for retrospective studies on archival material.
在过去几年中，已经发表了许多关于粒细胞-巨噬细胞集落刺激因子（GM-CSF）受体的研究，重点是分子结构、功能和分布。然而，据我们所知，尚未描述在福尔马林固定石蜡包埋组织学切片上检测GM-CSF受体的方法。
设计了一种基于非同位素原位杂交（ISH）的方法，使用一个21个碱基的反义DNA寡核苷酸探针，其3'端用洋地黄毒苷11-dUTP标记。该探针应用于20例常规处理的骨髓活检组织，这些组织被视为阳性对照。
在髓系细胞、红系祖细胞和罕见的巨核细胞中可见杂交信号。
非同位素原位杂交是评估GM-CSF受体表达的现有方法的一种替代方法；由于它适用于常规处理的样本，它可被视为一种有用的工具，用于诊断接受GM-CSF治疗患者肿瘤中的GM-CSF受体，并用于对存档材料的回顾性研究。
