Yoshida J, Mizuno M, Yagi K
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
J Neurooncol. 1994;19(3):269-74. doi: 10.1007/BF01053281.
We have been devoting our efforts to develop the useful liposomes that have high potentials of gene transfer and we aim human gene therapy for malignant glioma with cytokine genes. In our previous study, we prepared our original reverse-phase evaporation vesicles (REV) by an improved procedure of reverse-phase evaporation method. However, this procedure was very complicate. In this paper, a simple procedure for the preparation of cationic multilamellar vesicles (MLV) was introduced, and efficient expression and growth-inhibitory effect of MLV with entrapped human interferon-beta gene to glioma cells was comparable to that obtained with REV. Considering the present experiments, MLV seem to be more preferable for clinical application.
我们一直致力于开发具有高基因转移潜力的有用脂质体，并旨在利用细胞因子基因对恶性胶质瘤进行人类基因治疗。在我们之前的研究中，我们通过改进的反相蒸发法制备了我们原创的反相蒸发囊泡（REV）。然而，这个过程非常复杂。本文介绍了一种制备阳离子多层囊泡（MLV）的简单方法，并且包封了人干扰素-β基因的MLV对胶质瘤细胞的高效表达和生长抑制作用与REV相当。考虑到目前的实验，MLV似乎更适合临床应用。
