Westphal M, Stavrou D, Nausch H, Valdueza J M, Herrmann H D
Department of Neurosurgery, University Hospital Eppendorf, Hamburg, Germany.
J Neurosci Res. 1994 Aug 15;38(6):698-704. doi: 10.1002/jnr.490380612.
Cultured human neurocytoma cells from two neurosurgical patients were analysed for their immunocytochemical staining patterns and growth characteristics. In both cases, the cells stained positive for glial acidic fibrillary protein (GFAP) within one day of tissue culture in medium, with and without fetal calf serum, whereas the histological tumor specimens were negative. Both cases contained cells concomitantly expressing GFAP and synaptophysin (SNP) in the primary cultures. Epidermal growth factor (EGF) was mitogenic for the cultured cells but not platelet derived growth factor alpha (PDGF AA) or nerve growth factor (NGF). It is concluded that the human neurocytomas may represent neoplasms of a pluripotent neuroglial cell which can provide an interesting model to study the determinants for human glial/neuronal differentiation in vitro.
对两名神经外科患者的培养人神经细胞瘤细胞进行了免疫细胞化学染色模式和生长特性分析。在这两种情况下，细胞在添加和不添加胎牛血清的培养基中进行组织培养一天内，胶质酸性纤维蛋白（GFAP）染色呈阳性，而组织学肿瘤标本为阴性。在原代培养中，这两种情况的细胞均同时表达GFAP和突触素（SNP）。表皮生长因子（EGF）对培养细胞有促有丝分裂作用，但血小板衍生生长因子α（PDGF AA）或神经生长因子（NGF）则无此作用。得出的结论是，人类神经细胞瘤可能代表多能神经胶质细胞的肿瘤，它可以为体外研究人类神经胶质/神经元分化的决定因素提供一个有趣的模型。
