Yoshie H, Tobise K, Onodera S
First Department of Internal Medicine, Asahikawa Medical College, Japan.
Jpn Circ J. 1994 Nov;58(11):855-65. doi: 10.1253/jcj.58.855.
We investigated the changes in the membranous beta-adrenoceptor-adenylate cyclase system in the right ventricle, left ventricle and interventricular septum during the progress of monocrotaline-induced right ventricular hypertrophy and failure. The beta-adrenoceptor density was decreased in hypertrophied right ventricle 2 to 4 weeks after treatment. When the rats showed symptoms of right ventricular failure 4 weeks after treatment, the beta-adrenoceptor density was decreased in the interventricular septum. Both basal and forskolin-stimulated adenylate cyclase activities were decreased in the right ventricle at 3 and 4 weeks, and in the interventricular septum at 4 weeks, after treatment, which indicates that the catalytic activity of adenylate cyclase is reduced. Changes in isoproterenol plus Gpp (NH) p- or sodium fluoride-stimulated adenylate cyclase activity were generally similar to those in basal activity. These data indicate that a chamber-specific decrease in beta-adrenoceptor density begins in the early stages of right ventricular hypertrophy, and that beta-adrenoceptor density and adenylate cyclase activity in the interventricular septum are decreased in the advanced stages of heart failure in monocrotaline-treated rats.
我们研究了在野百合碱诱导的右心室肥大和衰竭过程中，右心室、左心室和室间隔膜性β-肾上腺素能受体-腺苷酸环化酶系统的变化。治疗后2至4周，肥厚的右心室中β-肾上腺素能受体密度降低。当大鼠在治疗后4周出现右心室衰竭症状时，室间隔中的β-肾上腺素能受体密度降低。治疗后3周和4周，右心室以及4周时室间隔中基础和福斯高林刺激的腺苷酸环化酶活性均降低，这表明腺苷酸环化酶的催化活性降低。异丙肾上腺素加Gpp（NH）p或氟化钠刺激的腺苷酸环化酶活性变化通常与基础活性变化相似。这些数据表明，β-肾上腺素能受体密度的室腔特异性降低始于右心室肥大的早期阶段，并且在野百合碱处理的大鼠心力衰竭晚期，室间隔中的β-肾上腺素能受体密度和腺苷酸环化酶活性降低。
