de Lecea L, Soriano E, Criado J R, Steffensen S C, Henriksen S J, Sutcliffe J G
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Brain Res Mol Brain Res. 1994 Sep;25(3-4):286-96. doi: 10.1016/0169-328x(94)90164-3.
We isolated a cDNA clone, named BSPL, that encodes a brain-specific dipeptidyl peptidase-like protein with 30% identity and 50% similarity to CD26, a lymphocyte membrane antigen involved in T-cell activation. BSPL lacks, however, the catalytic residue responsible for peptidase activity. The expression of BSPL is widespread throughout the CNS but restricted to neurons under normal conditions. Twenty-four hours after injection of kainic acid into the hippocampus, a dramatic increase in the concentration of BSPL mRNA was detected by in situ hybridization in the CA3 region of the injected hemisphere as compared with the contralateral hemisphere or sham-injected animals. An increase in the steady-state level of BSPL mRNA concentration was also found following tetanic stimulation of the perforant path to produce LTP in granule cells of the dentate gyrus. Hybridization signals could be detected in dendritic processes of pyramidal neurons and in some glial cells upon either type of stimulation. These data suggest that BSPL may be involved in synaptic plasticity.
我们分离出一个名为BSPL的cDNA克隆，它编码一种脑特异性二肽基肽酶样蛋白，与CD26（一种参与T细胞活化的淋巴细胞膜抗原）具有30%的同一性和50%的相似性。然而，BSPL缺乏负责肽酶活性的催化残基。BSPL的表达在整个中枢神经系统中广泛存在，但在正常情况下仅限于神经元。向海马体注射红藻氨酸24小时后，与对侧半球或假注射动物相比，通过原位杂交在注射半球的CA3区域检测到BSPL mRNA浓度显著增加。在对穿通路径进行强直刺激以在齿状回颗粒细胞中产生长时程增强（LTP）后，也发现BSPL mRNA浓度的稳态水平有所增加。在任何一种刺激下，在锥体神经元的树突过程和一些神经胶质细胞中都能检测到杂交信号。这些数据表明BSPL可能参与突触可塑性。
