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[不同核DNA水平的变形虫对高温和乙醇作用的抗性]

[The resistance of Amoeba proteus amebae with different levels of nuclear DNA to the action of high temperature and ethyl alcohol].

作者信息

Sopina V A, Afon'kin S Iu

出版信息

Tsitologiia. 1994;36(1):111-6.

PMID:7809957
Abstract

Thermotolerance and resistance to ethyl alcohol were investigated in Amoeba proteus of polyploid series. Thermoresistance of these amoebae was determined by measuring the mean time of survival at the lethal temperature (43 degrees C). The resistance to ethyl alcohol was estimated by the mean percentage of individuals that survived following a 5-minute treatment with 7.5% ethyl alcohol. Neither thermotolerance, nor ethyl alcohol resistance were found to depend on the amount of nuclear DNA. It was also established that colchicine did not induce a hereditary instability of thermoresistance in amoebae.

摘要

对多倍体系列的变形虫进行了耐热性和对乙醇耐受性的研究。通过测量在致死温度(43摄氏度)下的平均存活时间来确定这些变形虫的耐热性。通过用7.5%乙醇处理5分钟后存活个体的平均百分比来估计对乙醇的耐受性。结果发现,耐热性和对乙醇的耐受性均不取决于核DNA的含量。还证实秋水仙碱不会在变形虫中诱导耐热性的遗传不稳定性。

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