Kudriavtseva M V, Sakuta G A, Emel'ianov A V, Sleptsova L A, Skorina A D, Kudriavtsev B N
Tsitologiia. 1994;36(2):200-10.
Reversibility of hepatocyte functional activity is shown by cytofluorometric and microbiochemical methods in human and rat liver during postcirrhosis rehabilitation. Contents of the total glycogen and its fractions in liver cells were defined on smears of isolated hepatocytes obtained from the live puncture liver biopsies. A double increase of glycogen level is shown, in average, in hepatocytes during experimental liver cirrhosis in rats. At the same time, a relative content of the hard soluble fraction of glycogen increases by 5-8 times. The glycogen level falls to reach the norm already within one month after shutting off the pathogenic influence. However, in some animals after 6 months this level becomes even lower than the normal one. Again, the ratio between the hard soluble fraction and light one remains. In men with cirrhosis glycogenosis of hepatocytes can be expressed in greater degree (increase by 4-5 times): it depends on the illness heaviness. Further changes in glycogen content depend on the pathological process development. Under experimental cirrhosis the activity of glucose-6-phosphatase decreases by 4.3 times. Within one month after stopping the pathogenic influence the activity of this enzyme increases by 3-4 times, but later, in 6 months, it decreases to reach 55-65% of the norm. No actual changes were observed in the activities of other enzymes.
细胞荧光测定法和微生物化学方法表明，在肝硬化后康复过程中，人和大鼠肝脏的肝细胞功能活性具有可逆性。通过对经肝穿刺活检获取的分离肝细胞涂片进行分析，确定了肝细胞中总糖原及其各组分的含量。结果显示，在实验性肝硬化大鼠的肝细胞中，糖原水平平均增加了两倍。与此同时，糖原难溶性组分的相对含量增加了5至8倍。在消除致病因素后的一个月内，糖原水平降至正常范围。然而，在一些动物中，6个月后该水平甚至低于正常水平。同样地，难溶性组分与易溶性组分之间的比例依然存在。在患有肝硬化糖原贮积症男性患者中，肝细胞糖原水平的升高更为明显（增加4至5倍），这取决于疾病的严重程度。糖原含量的进一步变化取决于病理过程的发展。在实验性肝硬化过程中，葡萄糖-6-磷酸酶的活性降低了4.3倍。在停止致病因素影响后的一个月内，该酶的活性增加了3至4倍，但在6个月后，其活性降至正常水平的55%至65%。未观察到其他酶的活性有实际变化。
