Wayne L G, Sramek H A
Tuberculosis Research Laboratory, U.S. Department of Veterans Affairs Medical Center, Long Beach, California 90822.
Antimicrob Agents Chemother. 1994 Sep;38(9):2054-8. doi: 10.1128/AAC.38.9.2054.
Very abrupt exposure to anaerobic conditions has a lethal effect on actively growing cultures of Mycobacterium tuberculosis. However, incubation under conditions in which oxygen is depleted gradually causes M. tuberculosis to shift down from active replication to dormancy. The dormant bacilli are resistant to the bactericidal effects of anaerobiosis and also exhibit partial or complete resistance to the bactericidal effects of isoniazid and rifampin. On the other hand, metronidazole, a drug specific for anaerobes, kills dormant tubercle bacilli under anaerobic conditions, but it has no effect on actively growing aerobic cultures. The lethal effect of metronidazole under anaerobic conditions is enhanced by rifampin. The possible implications of these findings on the phenomenon of latency in tuberculosis are discussed.
突然暴露于无氧条件下对活跃生长的结核分枝杆菌培养物具有致死作用。然而，在氧气逐渐耗尽的条件下培养会使结核分枝杆菌从活跃复制转变为休眠状态。休眠杆菌对无氧环境的杀菌作用具有抗性，并且对异烟肼和利福平的杀菌作用也表现出部分或完全抗性。另一方面，甲硝唑是一种针对厌氧菌的药物，在无氧条件下可杀死休眠结核杆菌，但对活跃生长的需氧培养物没有作用。利福平可增强甲硝唑在无氧条件下的致死作用。本文讨论了这些发现对结核病潜伏现象可能产生的影响。
