Miyauchi A, Kuroki Y, Fukase M, Fujita T, Chihara K, Shiozawa S
Department of Medicine, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1547-55. doi: 10.1006/bbrc.1994.2843.
We analyzed c-fos mRNA expression by northern blotting analysis in chicken osteoclast precursors which spontaneously differentiate to multinucleated osteoclasts in 5-6 days. Osteoclast precursors as well as mature multinucleated osteoclasts showed constitutive expression of c-fos mRNA which is not found in osteoblasts. The c-fos expression was enhanced transiently by serum, dibutyryl cAMP (10(-4) M) and phorbol 12-myristate 13-acetate (TPA) (5 x 10(-7) M). To clarify the role of c-fos in osteoclast differentiation, c-fos DNA was transfected into osteoclast precursors. Greater than 2 fold increases in tartrate resistant acid phosphatase (TRAP) and bone resorptive activity were observed in the transfected cells compared to controls 3 days after transfection, suggesting that prolonged expression of c-fos caused enhanced osteoclast differentiation.
我们通过Northern印迹分析，对鸡破骨细胞前体中c-fos mRNA的表达进行了分析。这些破骨细胞前体在5-6天内可自发分化为多核破骨细胞。破骨细胞前体以及成熟的多核破骨细胞均显示出c-fos mRNA的组成性表达，而成骨细胞中未发现这种表达。血清、二丁酰环磷腺苷（10(-4) M）和佛波酯（TPA）（5×10(-7) M）可使c-fos的表达短暂增强。为阐明c-fos在破骨细胞分化中的作用，将c-fos DNA转染至破骨细胞前体中。转染3天后，与对照组相比，转染细胞中的抗酒石酸酸性磷酸酶（TRAP）和骨吸收活性增加了2倍以上，这表明c-fos的持续表达导致破骨细胞分化增强。
