Jounaïdi Y, Guzelian P S, Maurel P, Vilarem M J
INSERM U-128, CNRS, Montpellier, France.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1741-7. doi: 10.1006/bbrc.1994.2870.
Two genomic clones containing the 3'-end of CYP3A7, the whole 5'-flanking region plus the first exon of CYP3A5 were isolated from two different human genomic banks and sequenced. The 5'-flanking region of CYP3A5, from nucleotide -1 to -1434, was 60% and 59% similar with the corresponding regions of CYP3A4 and CYP3A7, respectively. However, the similarity increased to 74% when regions from nucleotide -1 to -700 were compared and dropped to less than 42% for regions upstream from -700. The CYP3A5 promoter contains a CATA box instead of the typical TATA box and a basic transcription element (BTE). Other consensus sequences previously found in CYP3A4 and CYP3A7 were also identified in the 5'-flanking of CYP3A5 from -1 to -700. Both CYP3A5 and CYP3A7 genes appear to be tandemly associated and transcribed in the same direction on chromosome 7.
从两个不同的人类基因组文库中分离出两个基因组克隆，其中一个包含CYP3A7的3'末端，另一个包含CYP3A5完整的5'侧翼区域加上第一个外显子，并进行了测序。CYP3A5的5'侧翼区域，从核苷酸-1到-1434，与CYP3A4和CYP3A7的相应区域分别有60%和59%的相似性。然而，当比较从核苷酸-1到-700的区域时，相似性增加到74%，而对于-700上游的区域，相似性降至42%以下。CYP3A5启动子包含一个CATA盒而不是典型的TATA盒以及一个基本转录元件（BTE）。先前在CYP3A4和CYP3A7中发现的其他共有序列也在CYP3A5从-1到-700的5'侧翼区域中被鉴定出来。CYP3A5和CYP3A7基因似乎串联相关，并在7号染色体上以相同方向转录。
