Yang Q, Paskind M, Bolger G, Thompson W J, Repaske D R, Cutler L S, Epstein P M
Department of Pharmacology, University of Connecticut Health Center, Farmington 06030.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1850-8. doi: 10.1006/bbrc.1994.2886.
A cDNA clone for cyclic GMP Stimulated Phosphodiesterase (cGSPDE; PDE2) was isolated from a rat brain cDNA library. The cDNA has an open reading frame which encodes a protein of 928 amino acids of which 829 are identical with the reported bovine adrenal gland cGSPDE cDNA (Sonnenburg, W.K., Mullaney, P.J., and Beavo, J.A. (1991) J. Biol. Chem. 266, 17655-17661). Although the overall homology of these two cDNAs is high, they are distinctly different in their 5' ends, with the N-terminal 37 amino acids of the rat brain protein showing no homology with the N-terminal end of the bovine adrenal protein. Hydrophilicity plots show that in contrast to the bovine adrenal cGSPDE, the N-terminal end of the rat brain cGSPDE is highly hydrophobic. Isolation and analysis of a genomic clone for cGSPDE from a rat genomic library shows the presence of an exon/intron junction at the Gln39 codon. The cGSPDE cDNA we have isolated and that of Sonnenburg et al. represent alternatively spliced mRNA products from the same gene, with the brain isoform designed to be targeted to membranes.
从大鼠脑cDNA文库中分离出一个编码环鸟苷酸刺激的磷酸二酯酶（cGSPDE；PDE2）的cDNA克隆。该cDNA具有一个开放阅读框，编码一个由928个氨基酸组成的蛋白质，其中829个氨基酸与已报道的牛肾上腺cGSPDE cDNA相同（索嫩堡，W.K.，穆拉尼，P.J.，和贝沃，J.A.（1991年）《生物化学杂志》266，17655 - 17661）。尽管这两个cDNA的总体同源性很高，但它们在5'端明显不同，大鼠脑蛋白的N端37个氨基酸与牛肾上腺蛋白的N端没有同源性。亲水性图谱显示，与牛肾上腺cGSPDE相反，大鼠脑cGSPDE的N端具有高度疏水性。从大鼠基因组文库中分离和分析cGSPDE的基因组克隆表明，在第39位谷氨酰胺密码子处存在一个外显子/内含子连接。我们分离出的cGSPDE cDNA与索嫩堡等人的cDNA代表来自同一基因的选择性剪接的mRNA产物，脑异构体被设计为靶向细胞膜。
