Moreno C, Garrigó M, Sánchez F, Coll P
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Enferm Infecc Microbiol Clin. 1994 May;12(5):241-5.
The usefulness of the microscopic examination of Bactec 12B and 13A growth medium as a method for the possible identification of M. tuberculosis complex, M. avium complex, M. xenopi, and M. kansasii was performed out to guide the selection of different genetic identification probes and, in the case of M. xenopi, the selection of the temperature of subcultures incubation.
Upon detection of an index of growth greater than 100 in Bactec tubes, staining was performed by the Ziehl-Neelsen technique. On the basis of the morphology observed, the possible identification was performed by genetic probes. Subcultures were used for definitive identification.
Three hundred forty-four positive samples were studied by radiometric technique. A total of 190 strains were identified as M. tuberculosis, 88 strains as M. avium-intracellulare (MAI), 33 strains as M. xenopi, 14 strains as M. kansasii and 19 strains were identified as: M. gordonae (10), unpigmented rapid growth microbacteria (7), and M. simiae (2). Sensitivity, specificity, positive predictive value, and negative predictive value were 97.9%, 95.4%, 96.4%, and 97.3%, respectively for M. tuberculosis complex, 84.0%, 99.2%, 97.3% 94.7% for M. avium complex; 63.6%, 98.3%, 80.7%, 96.2% for M. xenopi; 35.7%, 98.1%, 45.5% 97.2% for M. kansasii.
The morphology of M. tuberculosis complex examined in the radiometric system in useful to differentiate this species from other microbacteria (MOTT), allowing the selection of specific probe used. Within the MOTT, M. avium complex also has morphological characteristics which are useful for its differentiation, the morphology usually described for the remaining species was frequently not observed.
对Bactec 12B和13A生长培养基进行显微镜检查，以此作为一种可能鉴定结核分枝杆菌复合群、鸟分枝杆菌复合群、偶发分枝杆菌和堪萨斯分枝杆菌的方法，旨在指导不同基因鉴定探针的选择，对于偶发分枝杆菌，还可指导传代培养孵育温度的选择。
在Bactec管中检测到生长指数大于100后，采用萋-尼氏技术进行染色。根据观察到的形态，通过基因探针进行可能的鉴定。传代培养用于最终鉴定。
采用放射性技术研究了344份阳性样本。共鉴定出190株结核分枝杆菌、88株鸟分枝杆菌-胞内分枝杆菌（MAI）、33株偶发分枝杆菌、14株堪萨斯分枝杆菌，另有19株鉴定为：戈登分枝杆菌（10株）、无色素快速生长微生物（7株）和猿分枝杆菌（2株）。结核分枝杆菌复合群的敏感性、特异性、阳性预测值和阴性预测值分别为97.9%、95.4%、96.4%和97.3%；鸟分枝杆菌复合群分别为84.0%、99.2%、97.3%和94.7%；偶发分枝杆菌分别为63.6%、98.3%、80.7%和96.2%；堪萨斯分枝杆菌分别为35.7%、98.1%、45.5%和97.2%。
在放射性系统中检查的结核分枝杆菌复合群形态有助于将该菌种与其他微生物（非结核分枝杆菌）区分开来，从而可以选择特定的探针。在非结核分枝杆菌中,鸟分枝杆菌复合群也具有有助于其鉴别的形态特征，而通常描述的其余菌种的形态特征却常常未观察到。
