Dufrenne J, Tatini S, Notermans S
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Int J Food Microbiol. 1994 Sep;23(1):111-6. doi: 10.1016/0168-1605(94)90226-7.
Spores of four different strains of Bacillus cereus were stored on silicagel at 22 degrees C and in physiological saline solution at -20 degrees C for a period of 260 days. At different intervals the spores were tested for survival, heat sensitivity and capacity to germinate. There was no clear change in any of the parameters tested, so storage on silicagel can be a good alternative for storage as a frozen suspension. Spores stored in this way can easily be exchanged for reference material and used for microbiological challenge testing.
将四种不同蜡样芽孢杆菌菌株的孢子分别在22摄氏度下保存在硅胶上，并在-20摄氏度的生理盐溶液中保存260天。在不同时间间隔对孢子进行存活、热敏感性和发芽能力测试。所测试的任何参数均未出现明显变化，因此在硅胶上保存可作为冷冻悬浮液保存的良好替代方法。以这种方式保存的孢子可轻松作为参考材料进行交换，并用于微生物挑战性试验。
