Wiebe M G, Robson G D, Oliver S G, Trinci A P
School of Biological Sciences, University of Manchester, UK.
Microbiology (Reading). 1994 Nov;140 ( Pt 11):3015-21. doi: 10.1099/13500872-140-11-3015.
Variants (designated A23-S and A24-S) of the Quorn myco-protein fungus, Fusarium graminearum A3/5 were isolated from a series of glucose-limited cultures grown at a dilution rate of 0.18 h-1 for a combined total of 109 d. These variants had unchanged mycelial morphologies but, when grown in mixed culture with the parental strain (A3/5) in glucose-limited chemostat culture at 0.18 h-1, A23-S and A24-S had selection coefficients of 0.013 and 0.017 h-1, respectively, and supplanted A3/5. When a monoculture of A23-S was grown in a glucose-limited culture at a dilution rate of 0.18 h-1, the appearance of highly branched (so-called colonial) mutants was delayed compared with their appearance in chemostat cultures of the parental strain. Furthermore, when a monoculture of A24-S was grown in glucose-limited culture at 0.18 h-1, the appearance of colonial mutants was delayed even further. Thus, it is possible to isolate advantageous (relative to A3/5) variants of F. graminearum A3/5 which have unchanged mycelial morphologies, but in which the appearance of colonial mutants is delayed.
从一系列以0.18 h⁻¹的稀释率培养109天的葡萄糖限制培养物中分离出了禾谷镰刀菌A3/5（即Quorn霉菌蛋白真菌）的变体（命名为A23 - S和A24 - S）。这些变体的菌丝形态没有变化，但是，当它们与亲本菌株（A3/5）在葡萄糖限制的恒化器培养中以0.18 h⁻¹混合培养时，A23 - S和A24 - S的选择系数分别为0.013 h⁻¹和0.017 h⁻¹，并取代了A3/5。当A23 - S的单培养物在葡萄糖限制培养中以0.18 h⁻¹生长时，与亲本菌株在恒化器培养中的情况相比，高度分支（所谓的菌落状）突变体的出现延迟了。此外，当A24 - S的单培养物在葡萄糖限制培养中以0.18 h⁻¹生长时，菌落状突变体的出现延迟得更厉害。因此，有可能分离出具有不变菌丝形态的禾谷镰刀菌A3/5的优势（相对于A3/5）变体，但其菌落状突变体的出现会延迟。
