Corfe B M, Moir A, Popham D, Setlow P
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Microbiology (Reading). 1994 Nov;140 ( Pt 11):3079-83. doi: 10.1099/13500872-140-11-3079.
The gerB spore germination operon of Bacillus subtilis 168 is a homologue of the gerA spore germination operon. The expression and regulation of the gerB operon has been examined using a lacZ transcriptional fusion and the transcriptional start defined. The gerB operon is expressed during sporulation under the control of RNA polymerase containing the forespore-specific sigma factor, delta G. This is a further homology to the gerA operon, which is similarly regulated. It is predicted from the localization of expression and the encoded primary sequences that the GerB proteins are located at the inner spore membrane.
枯草芽孢杆菌168的gerB孢子萌发操纵子是gerA孢子萌发操纵子的同源物。已使用lacZ转录融合对gerB操纵子的表达和调控进行了研究，并确定了转录起始位点。gerB操纵子在芽孢形成过程中，在含有前芽孢特异性σ因子δG的RNA聚合酶的控制下表达。这是与gerA操纵子的又一同源性，gerA操纵子也受到类似的调控。根据表达定位和编码的一级序列预测，GerB蛋白位于孢子内膜。
