Dallinga-Thie G M, van Linde-Sibenius Trip M, Kock L A, De Bruin T W
Department of Medicine and Endocrinology, Utrecht University, The Netherlands.
Clin Chem. 1995 Jan;41(1):73-5.
Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles.
在此，我们报告一种改进的、简单的方法来确定人类载脂蛋白（apo）E基因型及其异构体apo E2、apo E3和apo E4。用包含氨基酸112和158多态性区域的特异性引物扩增基因组DNA。用Hhal消化产物产生独特的片段，这些片段在Meta-Phor琼脂糖而非聚丙烯酰胺上进行分离。所获得的独特DNA片段模式明确地鉴定了不同的apo E等位基因。
