用于蛋白质电动处理的多功能装置。

Multifunctional apparatus for electrokinetic processing of proteins.

作者信息

Horvath Z S, Corthals G L, Wrigley C W, Margolis J

机构信息

University of Technology of Sydney, NSW, Australia.

出版信息

Electrophoresis. 1994 Jul;15(7):968-71. doi: 10.1002/elps.11501501141.

DOI:10.1002/elps.11501501141

PMID:7813403

Abstract

New equipment (the "Gradiflow") has been designed and constructed to provide efficient large-scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.

摘要

新设备（“梯度流仪”）的设计和制造目的是基于电荷和/或尺寸差异、大分子浓缩以及电渗析，对大分子进行高效的大规模制备性分离。其功能示例包括在15分钟内分离血红蛋白（50毫克）和牛血清白蛋白（50毫克）的混合物（基于pH 6.8时的电荷差异）、基于尺寸从粗提物中纯化藻红蛋白，以及将血清蛋白分离成两个不同的尺寸类别。