Davis G E, Camarillo C W
Department of Pathology, Texas A&M University Health Science Center, College Station 77843-1114.
Exp Cell Res. 1995 Jan;216(1):113-23. doi: 10.1006/excr.1995.1015.
Basement membrane matrix is known to induce human endothelial cells to form cord-like structures that mimic those observed during early angiogenesis in vivo. Using this model, blocking antibody studies revealed a major role for the alpha 6 beta 1 integrin in cord formation. During this process, two alterations in the Matrigel structure were observed which suggested a mechanism for the precision of cord formation. First, Matrigel contracted and lifted off an agarose support and second, linear distortions became visible in the Matrigel that correspond to the migration pathways of endothelial cell processes. These pathways, which we have termed "matrix guidance pathways," appear to result from the generation of mechanical tension between endothelial cells. The above data support the concept that endothelial cell guidance during morphogenetic events could be controlled by the ability of these cells to exert mechanical forces on the surrounding extracellular matrix to create pathways for migration.
已知基底膜基质可诱导人内皮细胞形成索状结构，这些结构类似于在体内早期血管生成过程中观察到的结构。利用该模型，阻断抗体研究揭示了α6β1整合素在索状结构形成中的主要作用。在此过程中，观察到基质胶结构的两种变化，这提示了索状结构形成精确性的一种机制。首先，基质胶收缩并从琼脂糖支持物上抬起，其次，基质胶中出现了与内皮细胞突起迁移途径相对应的线性扭曲。这些途径，我们称之为“基质引导途径”，似乎是由内皮细胞之间产生的机械张力所致。上述数据支持这样一种概念，即在形态发生事件中内皮细胞的引导可能受这些细胞对周围细胞外基质施加机械力以创造迁移途径能力的控制。
