Huang M, Oppermann F B, Steinbüchel A
Department of Biology, Scihuan Normal University, Chengdu, China.
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50. doi: 10.1111/j.1574-6968.1994.tb07276.x.
The 2,3-butanediol dehydrogenase and the acetoin-cleaving system were simultaneously induced in Pseudomonas putida PpG2 during growth on 2,3-butanediol and on acetoin. Hybridization with a DNA probe covering the genes for the E1 subunits of the Alcaligenes eutrophus acetoin cleaving system and nucleotide sequence analysis identified acoA (975 bp), acoB (1020 bp), apoC (1110 bp), acoX (1053 bp) and adh (1086 bp) in a 6.3-kb genomic region. The amino acid sequences deduced from acoA, acoB, and acoC for E1 alpha (M(r) 34639), E1 beta (M(r) 37268), and E2 (M(r) 39613) of the P. putida acetoin cleaving system exhibited striking similarities to those of the corresponding components of the A. eutrophus acetoin cleaving system and of the acetoin dehydrogenase enzyme system of Pelobacter carbinolicus and other bacteria. Strong sequence similarities of the adh translational product (2,3-butanediol dehydrogenase, M(r) 38361) were obtained to various alcohol dehydrogenases belonging to the zinc- and NAD(P)-dependent long-chain (group I) alcohol dehydrogenases. Expression of the P. putida ADH in Escherichia coli was demonstrated. The aco genes and adh constitute presumably one single operon which encodes all enzymes required for the conversion of 2,3-butanediol to central metabolites.
在恶臭假单胞菌PpG2以2,3-丁二醇和乙偶姻为碳源生长期间，同时诱导产生了2,3-丁二醇脱氢酶和乙偶姻裂解系统。用覆盖产碱杆菌乙偶姻裂解系统E1亚基基因的DNA探针进行杂交，并通过核苷酸序列分析，在一个6.3 kb的基因组区域中鉴定出了acoA（975 bp）、acoB（1020 bp）、apoC（1110 bp）、acoX（1053 bp）和adh（1086 bp）。从恶臭假单胞菌乙偶姻裂解系统的acoA、acoB和acoC推导的氨基酸序列显示，E1α（相对分子质量34639）、E1β（相对分子质量37268）和E2（相对分子质量39613）与产碱杆菌乙偶姻裂解系统以及乙醇杆菌和其他细菌的乙偶姻脱氢酶系统的相应组分具有显著相似性。adh翻译产物（2,3-丁二醇脱氢酶，相对分子质量38361）与属于锌和NAD（P）依赖性长链（I组）醇脱氢酶的各种醇脱氢酶具有很强的序列相似性。证实了恶臭假单胞菌ADH在大肠杆菌中的表达。aco基因和adh可能构成一个单一操纵子，该操纵子编码将2,3-丁二醇转化为中心代谢物所需的所有酶。
