Ikai H, Yamamoto S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
FEMS Microbiol Lett. 1994 Dec 1;124(2):225-8. doi: 10.1016/0378-1097(94)90253-4.
The gene encoding L-2,4-diaminobutyrate decarboxylase (DABA DC) was cloned from Acinetobacter baumannii ATCC 19606. The gene was evidently under the control of its own promoter. Interestingly, the host carrying this clone also produced an appreciable amount of 1,3-diaminopropane. Restriction mapping and subsequent subcloning of the cloned insert localized the DABA DC gene within a 2.45-kb SphI/EcoRI fragment. For endogenous production of DAP, a 1.75-kb EcoRI/PstI region downstream from the DABA DC gene was further required. Southern blot hybridization revealed some heterogeneity in the DABA DC genes among other Acinetobacter species.
编码L-2,4-二氨基丁酸脱羧酶（DABA DC）的基因从鲍曼不动杆菌ATCC 19606中克隆得到。该基因显然受其自身启动子的控制。有趣的是，携带此克隆的宿主也产生了相当数量的1,3-二氨基丙烷。对克隆插入片段进行限制性图谱分析及随后的亚克隆，将DABA DC基因定位在一个2.45 kb的SphI/EcoRI片段内。为了内源性产生DAP，还需要DABA DC基因下游一个1.75 kb的EcoRI/PstI区域。Southern印迹杂交显示其他不动杆菌属物种的DABA DC基因存在一些异质性。
