Deshpande J V, Thampi P P
Government College of Pharmacy, Bangalore, India.
Indian J Exp Biol. 1994 Sep;32(9):672-3.
6-Amino penicillanic acid (6-APA) was condensed with D-alpha-phenyl glycine chloride in presence of E. coli NCIM 2563 to form ampicillin. At pH 5, 30% of 6-APA was converted to ampicillin during 1 hr incubation. When E. coli cells were immobilized in calcium alginate beads, the activity remained unaffected even after six batches of bioconversion. Inclusion of glutaraldehyde as multifunctional cross-linking agent, improved the stability of the biocatalyst beads.
在大肠杆菌NCIM 2563存在的情况下，6-氨基青霉烷酸（6-APA）与D-α-苯基甘氨酸氯化物缩合形成氨苄青霉素。在pH值为5时，孵育1小时期间，30%的6-APA转化为氨苄青霉素。当将大肠杆菌细胞固定在海藻酸钙珠中时，即使经过六批生物转化，其活性仍不受影响。加入戊二醛作为多功能交联剂，提高了生物催化剂珠的稳定性。
