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来自发酵乳杆菌的白喉棒状杆菌dtxR同源物的分子克隆、DNA序列分析及特性鉴定

Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR homolog from Brevibacterium lactofermentum.

作者信息

Oguiza J A, Tao X, Marcos A T, Martín J F, Murphy J R

机构信息

Area of Microbiology, Faculty of Biology, University of León, Spain.

出版信息

J Bacteriol. 1995 Jan;177(2):465-7. doi: 10.1128/jb.177.2.465-467.1995.

DOI:10.1128/jb.177.2.465-467.1995
PMID:7814338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176612/
Abstract

A homolog of the Corynebacterium diphtheriae dtxR gene was isolated from Brevibacterium lactofermentum. The product of the B. lactofermentum dtxR gene was immunoreactive with polyclonal anti-DtxR antibodies and functioned as an iron-activated repressor capable of regulating the expression of beta-galactosidase from a diphtheria tox promoter/operator transcriptional fusion in recombinant Escherichia coli. The extents of induction by increasing concentrations of the chelator 2,2'-dipyridyl were identical in cells expressing DtxR from either C. diphtheriae or B. lactofermentum.

摘要

从乳酸发酵短杆菌中分离出了与白喉棒状杆菌dtxR基因同源的基因。乳酸发酵短杆菌dtxR基因的产物与多克隆抗DtxR抗体发生免疫反应，并作为一种铁激活阻遏物发挥作用，能够调节重组大肠杆菌中来自白喉毒素启动子/操纵子转录融合体的β-半乳糖苷酶的表达。在表达来自白喉棒状杆菌或乳酸发酵短杆菌的DtxR的细胞中，随着螯合剂2,2'-联吡啶浓度的增加，诱导程度是相同的。

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本文引用的文献

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Characterization of an iron-regulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter.
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Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR).使白喉毒素阻遏基因（dtxR）失活的突变的特征分析。
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Cloning, sequence, and footprint analysis of two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron.对白喉棒状杆菌中受白喉毒素阻遏物（DtxR）和铁调控的两个启动子/操纵子的克隆、测序及足迹分析
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Determination of the minimal essential nucleotide sequence for diphtheria tox repressor binding by in vitro affinity selection.通过体外亲和选择确定白喉毒素阻遏物结合的最小必需核苷酸序列。
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Iron, DtxR, and the regulation of diphtheria toxin expression.铁、DtxR与白喉毒素表达的调控
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7
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10
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Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5897-901. doi: 10.1073/pnas.89.13.5897.