Heijink E, Bolhuis P A, de Wolff F A
Academic Medical Center, University of Amsterdam, The Netherlands.
J Neuropathol Exp Neurol. 1995 Jan;54(1):82-90. doi: 10.1097/00005072-199501000-00010.
The effect of 2,5-hexanedione (2,5-HD) on the distribution of the neurofilamental (NF) proteins and vimentin was examined in human neuroblastoma cell line SK-N-SH with immunocytochemical methods. Retinoic acid (10 microM) induced differentiation into neuronal cells resulting in the outgrowth of processes and synthesis of NF proteins in the majority of the cells. A minority (4%) differentiated as large fibroblasts. Cells were exposed to 0-10 mM 2,5-HD for 3 days. In neuronal cells a concentration-dependent accumulation of NF proteins was detected as a spherical structure in the perikaryon. Neurofilaments in differentiated SK-N-SH cells were more susceptible to 2,5-HD than NF in undifferentiated cells, as the effects were observed at much lower 2,5-HD concentrations. In contrast, no accumulation of vimentin was detected in the fibroblastic cells.
采用免疫细胞化学方法，在人神经母细胞瘤细胞系SK-N-SH中检测了2,5-己二酮（2,5-HD）对神经丝（NF）蛋白和波形蛋白分布的影响。视黄酸（10微摩尔）诱导细胞分化为神经元细胞，导致大多数细胞长出突起并合成NF蛋白。少数细胞（4%）分化为大的成纤维细胞。将细胞暴露于0-10毫摩尔的2,5-HD中3天。在神经元细胞中，检测到NF蛋白呈浓度依赖性地在核周积累为球形结构。与未分化细胞中的NF相比，分化的SK-N-SH细胞中的神经丝对2,5-HD更敏感，因为在低得多的2,5-HD浓度下就观察到了这种效应。相反，在成纤维细胞中未检测到波形蛋白的积累。
