Farmer J J, Hickman F W, Sikes J V
Lancet. 1975 Oct 25;2(7939):787-90. doi: 10.1016/s0140-6736(75)80004-3.
Two steps in the procedure for bacteriophage typing of Salmonella typhi have been automated. The culture inoculum was applied by flooding the surface of phage agar in a 150x20 mm petri dish and removing the excess liquid with a safety pipettor. This step replaced the older method of manually preparing up to 100 individual areas for inoculation. The number of bacteria per unit area was the same with both methods, so the automated method involved no change in the technique. The second automated step involved use of a bacteriophage applicator which simultaneously dispensed 59 uniform drops of bacteriophages onto the inoculated plate. The automated procedure has reduced personnel time by about 90% with no less in sensitivity or accuracy.
伤寒沙门氏菌噬菌体分型程序中的两个步骤已实现自动化。通过将培养接种物倾注在150×20毫米培养皿中的噬菌体琼脂表面，并用安全移液器去除多余液体来进行接种。这一步骤取代了以前手动制备多达100个单独接种区域的方法。两种方法每单位面积的细菌数量相同，因此自动化方法在技术上没有变化。第二个自动化步骤涉及使用噬菌体涂抹器，它能同时将59滴均匀的噬菌体滴加到接种平板上。自动化程序将人员时间减少了约90%，同时灵敏度和准确性并未降低。
