Feng H L, Yang Q Z, Sun Q Y, Qin P C, Liu J M
Department of Biological Science, Changchun Veterinary College, China.
Vet Rec. 1994 Sep 24;135(13):304-6. doi: 10.1136/vr.135.13.304.
The objective of this study was to examine the effects of different culture systems on the development of early bovine embryos in vitro. A total of 1089 oocytes were aspirated from 2 to 5 mm follicles of ovaries collected at a local abattoir; a high proportion of the oocytes matured in vitro were fertilised by spermatozoa capacitated with caffeine and heparin. Seven to eight hours after insemination, the oocytes were transferred into three in vitro systems: A, TCM 199 + 10 per cent fetal calf serum culture medium, B, coculture with a monolayer of granulosa cells and C, coculture with bovine oviductal epithelial cells. The results showed that the proportion of the early bovine embryos which overcame the block at eight to 16 cells and developed to the morula and blastocyst stages in system C was significantly higher than in systems A or B.
本研究的目的是检测不同培养系统对早期牛胚胎体外发育的影响。从当地屠宰场收集的卵巢2至5毫米卵泡中总共吸出1089个卵母细胞；体外成熟的卵母细胞中有很大比例被用咖啡因和肝素获能的精子受精。授精后7至8小时，将卵母细胞转移到三种体外系统中：A，TCM 199 + 10%胎牛血清培养基；B，与单层颗粒细胞共培养；C，与牛输卵管上皮细胞共培养。结果显示，在系统C中克服8至16细胞阻滞并发育到桑葚胚和囊胚阶段的早期牛胚胎比例显著高于系统A或B。
