Kadokami Y, Colgin M A, Yesgar P W, Lewis R V
University of Wyoming, Laramie.
Biotechniques. 1994 Sep;17(3):580-4.
An ultra-quick method for plasmid DNA extraction using the ScreenMax Plasmid Mini-prep Kit was optimized. Since the method has fewer steps than current methods, the entire process takes only 12 min for two sets of plasmid DNA extractions. DNA quality was excellent for further analyses, including DNA sequencing. The kit's new medium, MMB, provided 5 times the viable cell count and 7 times the dry cell weight compared with the conventional LB medium. The yield of plasmid DNA was 5 times better than from LB medium. From 200 microL of culture, the maximum yield was 7.5 micrograms. The ScreenMax Plasmid Mini-Prep is not only simple and economical but also safe because of its mild reagents. This new procedure is both reliable and reproducible.
优化了一种使用ScreenMax质粒小量制备试剂盒提取质粒DNA的超快速方法。由于该方法的步骤比现有方法少，两组质粒DNA提取的整个过程仅需12分钟。DNA质量非常适合进一步分析，包括DNA测序。与传统的LB培养基相比，该试剂盒的新型培养基MMB的活细胞计数提高了5倍，干细胞重量提高了7倍。质粒DNA的产量比LB培养基提高了5倍。从200微升培养物中，最大产量为7.5微克。ScreenMax质粒小量制备试剂盒不仅简单经济，而且因其试剂温和而安全。这种新方法既可靠又可重复。
