Hozumi K, Kondo M, Nozaki H, Kobori A, Nishimura T, Nishikawa S, Sugamura K, Habu S
Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
Int Immunol. 1994 Sep;6(9):1451-4. doi: 10.1093/intimm/6.9.1451.
The effects of IL-7 on the growth and differentiation of thymocytes were analyzed using murine fetal thymus organ cultures (FTOC) in the presence of mAbs specific for the conventional IL-7 receptor (IL-7R) and for the common gamma (gamma c) chain. In FTOC, the development of CD4-CD8- double-negative thymocytes to CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) cells was not completely blocked by adding these mAbs, although cell growth was reduced by the treatment. To define a developing stage sensitive to the mAbs, most immature thymocytes, Pgp-1+ c-kit+ cells, were cultured in 2-deoxyguanosine treated fetal thymus. In the presence of both mAbs in the culture, neither DP nor SP thymocytes developed whereas either of the mAbs partially blocked their development. These results indicate that the gamma c chain is involved in early T cell development as an indispensable subunit of the functional IL-7 receptor complex.
在存在针对传统白细胞介素-7受体（IL-7R）和共同γ（γc）链的单克隆抗体（mAb）的情况下，使用小鼠胎儿胸腺器官培养物（FTOC）分析了白细胞介素-7对胸腺细胞生长和分化的影响。在FTOC中，添加这些单克隆抗体并未完全阻断CD4-CD8-双阴性胸腺细胞向CD4+CD8+双阳性（DP）和CD4+或CD8+单阳性（SP）细胞的发育，尽管处理会降低细胞生长。为了确定对单克隆抗体敏感的发育阶段，将大多数未成熟胸腺细胞Pgp-1+c-kit+细胞培养在经2-脱氧鸟苷处理的胎儿胸腺中。在培养物中同时存在两种单克隆抗体时，DP和SP胸腺细胞均未发育，而任何一种单克隆抗体均可部分阻断其发育。这些结果表明，γc链作为功能性IL-7受体复合物不可或缺的亚基，参与早期T细胞发育。
