Tooze S A, Hollinshead M, Dittié A S
Imperial Cancer Research Institute, London, UK.
Biochimie. 1994;76(3-4):271-6. doi: 10.1016/0300-9084(94)90157-0.
Several events occur during secretory granule maturation in endocrine and neuronal cells, one of the most important being the processing of prohormones. In addition, secretory granules undergo several changes during storage and maturation within the cell. We have been investigating the maturation of secretory granules in the neuroendocrine cell line PC12. Our working hypothesis postulates that fusion of newly budded secretory granules occurs during maturation and results in a larger secretory granule. We have been investigating the kinetics and specificity of the prohormone processing enzymes towards secretogranin II. Processing of secretogranin II must occur predominantly in the maturing secretory granule and could be used as a method to monitor secretory granule maturation.
在内分泌细胞和神经元细胞的分泌颗粒成熟过程中会发生若干事件，其中最重要的之一是激素原的加工。此外，分泌颗粒在细胞内储存和成熟期间会经历若干变化。我们一直在研究神经内分泌细胞系PC12中分泌颗粒的成熟过程。我们的工作假设假定新出芽的分泌颗粒在成熟过程中发生融合，从而产生更大的分泌颗粒。我们一直在研究激素原加工酶对分泌粒蛋白II的动力学和特异性。分泌粒蛋白II的加工必定主要发生在成熟的分泌颗粒中，并且可作为监测分泌颗粒成熟的一种方法。
