Rodina E V, Cherkasov A V, Nedospasov A A
Biokhimiia. 1994 Oct;59(10):1544-59.
ANSA-analysis was used for characterization of proteases and their mixtures, such as snake venoms. The method is based on the cleavage by proteases of mixtures of competing chromogenic substrates containing substituted aminonaphtalenesulfonamide (ANSA) detectable groups. All detectable ANSA groups in the substrate mixtures have non-identical modifiers, one or two substituents in the sulfonamide fragment and can be determined by chromatographic methods. To identify venoms, a mixture of six peptide substrates cleaved at the Arg-ANSA bond was proposed. Hydrolysis of this substrate mixture catalyzed by the venoms of different Crotalidae and Viperidae species gave characteristic chromatograms (ANSA spectra) for each tested sample. A method for quantitative description of differences in ANSA spectra has been proposed. Each ANSA spectrum can be presented as a vector going from the origin of the coordinated axes to a point in an n-dimensional space (n is the number of assayed ANSA products of proteolysis) with peak squares of corresponding ANSA as coordinates. The similarity between two ANSA spectra will then be characterized by angle between their vectors.
ANSA分析用于表征蛋白酶及其混合物，如蛇毒。该方法基于蛋白酶对含有可检测取代氨基萘磺酰胺（ANSA）基团的竞争性生色底物混合物的裂解。底物混合物中所有可检测的ANSA基团具有不同的修饰基团，即磺酰胺片段中有一个或两个取代基，并且可以通过色谱方法测定。为了鉴定蛇毒，有人提出了一种在Arg-ANSA键处裂解的六种肽底物的混合物。不同蝰蛇科和蝰蛇科物种的毒液催化该底物混合物的水解，为每个测试样品给出了特征色谱图（ANSA光谱）。已经提出了一种定量描述ANSA光谱差异的方法。每个ANSA光谱可以表示为从坐标轴原点到n维空间中一个点的向量（n是蛋白水解测定的ANSA产物数量），以相应ANSA的峰面积为坐标。然后，两个ANSA光谱之间的相似性将由它们向量之间的夹角来表征。
