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[紫外线诱导HeLa细胞DNA-蛋白质交联的研究]

[Study of DNA-protein crosslinks induced by UV light in Hela cells].

作者信息

Kolomiĭtseva G Ia, Kurochkina L P, Bobkova E A

出版信息

Biokhimiia. 1994 Aug;59(8):1192-7.

PMID:7819405
Abstract

UV-Irradiation (lambda = 254 nm) of Hela cells in the monolayer causes a dose-dependent formation of DNA-protein crosslinks (DPC). The time-dependent protein increase unaccompanied by a change in the complex yield was observed for the fraction of the DNA-protein complex passing into the aqueous phase by phenol extraction, when the cells were transferred to a fresh culture medium after UV-irradiation. These data suggest that DPC do not repair, while DNA or the protein within the complex can firmly bind additional quantity of protein during the post-irradiation period.

摘要

对单层培养的Hela细胞进行紫外线(波长 = 254 nm)照射会导致剂量依赖性的DNA-蛋白质交联(DPC)形成。当细胞在紫外线照射后转移到新鲜培养基中时,通过苯酚抽提进入水相的DNA-蛋白质复合物部分出现了随时间增加的蛋白质,且复合物产量没有变化。这些数据表明DPC不会修复,而复合物中的DNA或蛋白质在照射后时期可以牢固结合额外数量的蛋白质。

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