大鼠着床部位两种溶酶体酶的核内存在情况。
Nuclear presence of two lysosomal enzymes in rat implantation sites.
作者信息
Duran-Reyes G, Diaz-Flores M, Castillo S, Hicks J J
机构信息
División de Biologia de la Reproducción, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico, D.F., Mexico.
出版信息
Int J Fertil Menopausal Stud. 1994 Sep-Oct;39(5):299-303.
OBJECTIVE
To test the Szego hypothesis of increased beta-glucuronidase and acid phosphatase activities in hormone target tissues.
METHODS
The presence of beta-glucuronidase and acid phosphatase activities in nuclear subcellular fractions obtained from decidual (implantation site) and stromal (nonimplantation zone) tissues was demonstrated by both biochemical measurements and ultramicrographic analysis utilizing a histochemical reaction.
RESULTS
Acid phosphatase was almost twice as abundant in nuclei and lysosomes of epithelial cells (implantation sites), and beta-glucuronidase also was significantly more active in nuclei from epithelial and decidual tissues than in nonimplantation tissue.
CONCLUSION
Our results, utilizing the implantation process as experimental model, support the Szego hypothesis of the lysosomal role in hormonal mechanisms of action.
目的
验证塞戈关于激素靶组织中β-葡萄糖醛酸酶和酸性磷酸酶活性增加的假说。
方法
通过生化测量和利用组织化学反应的超微图像分析，证实了从蜕膜（着床部位）和基质（非着床区）组织获得的核亚细胞组分中存在β-葡萄糖醛酸酶和酸性磷酸酶活性。
结果
酸性磷酸酶在上皮细胞（着床部位）的细胞核和溶酶体中的含量几乎是其两倍，并且β-葡萄糖醛酸酶在上皮和蜕膜组织细胞核中的活性也明显高于非着床组织。
结论
我们以着床过程作为实验模型的研究结果支持了塞戈关于溶酶体在激素作用机制中作用的假说。
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