Lawrence P A, Johnston P, Vincent J P
MRC Laboratory of Molecular Biology, Cambridge, UK.
Development. 1994 Dec;120(12):3355-9. doi: 10.1242/dev.120.12.3355.
By means of nuclear transplantations, we make mosaics in which largely wingless- embryos contain patches of wingless+ cells. In these genetic mosaics, using a standard assay for wingless function (the maintenance of engrailed expression), we uncover an induction across germ layers: Wingless made in the mesoderm can sustain engrailed expression in the ectoderm. This result makes clear that Wingless is expressed in the mesoderm until at least one hour after gastrulation and may function in this germ layer in the wild type.
通过核移植，我们制造了嵌合体，其中大部分无翅胚胎包含无翅+细胞斑块。在这些基因嵌合体中，我们使用一种用于检测无翅功能的标准方法（维持 engrailed 表达），发现了跨胚层的诱导作用：中胚层产生的无翅能够维持外胚层中 engrailed 的表达。这一结果清楚地表明，无翅在原肠胚形成后至少一小时内在中胚层中表达，并且在野生型中可能在这个胚层中发挥作用。
