René F, Hindelang C, Stoeckel M E, Félix J M
Laboratoire de Physiologie générale, URA CNRS No. 1446, Strasbourg, France.
Mol Cell Endocrinol. 1994 Oct;105(1):65-75. doi: 10.1016/0303-7207(94)90036-1.
The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13-E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17-E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed.
通过原位杂交（ISH）研究了大鼠垂体发育过程中糖皮质激素和D2多巴胺受体（GR和D2R）的表达。在早期阶段，即E13 - E14，整个拉特克囊（RP）中GR mRNA有微弱的特异性信号，而随后，从E17 - E18开始，强标记仅限于前叶（AL）和神经叶（NL）。同时，D2R mRNA出现在中间叶（IL），并且用特异性探针可检测到D2R的长异构体（D2R 444）。在出生后阶段，直至成年，使用传统ISH技术在IL中始终检测不到GR mRNA（如果存在的话）。这些数据表明，糖皮质激素可能在AL的促肾上腺皮质激素细胞中对POMC基因表达进行早期调节，而多巴胺可能在IL的促黑素细胞中进行早期调节。本文还讨论了早在E17时多巴胺（DA）对IL中GR mRNA进行负调节的可能性。
