Rachinsky A, Zhang J, Tobe S S
Zoologisches Institut, Universität Tübingen, Germany.
Mol Cell Endocrinol. 1994 Oct;105(1):89-96. doi: 10.1016/0303-7207(94)90039-6.
The effects of pharmacological agents that interfere with the 1,4,5-inositol trisphosphate (IP3)/diacylglycerol (DAG) pathway on juvenile hormone (JH) biosynthesis by corpora allata (CA) of the cockroach Diploptera punctata have been investigated. These effects were assessed in the presence of the inhibitory neuropeptides, allatostatins, with a view to elucidating the pathway for signal transduction in the inhibition of JH biosynthesis. Treatment of CA with inhibitors of DAG kinase to elevate the concentration of DAG within the CA cells, resulted in a significant, dose-dependent decrease in JH biosynthesis. Simultaneous treatment of glands with both DAG kinase inhibitors and allatostatins further enhanced this effect, suggesting that DAG is an intermediate in the allatostatin-induced inhibition of JH production. The inhibitory actions of the phorbol ester activator of PKC, PDBu, or of allatostatin on JH biosynthesis were partially blocked by pre-incubating the CA with PKC inhibitors. Treatment of CA with the calcium-mobilizing drug thapsigargin resulted in a significant stimulation in JH biosynthesis in glands from mated females producing JH at high rates. Thapsigargin was also able to reverse the effect of allatostatins in high-activity mated CA. This suggests an involvement of the other product of phosphoinositide hydrolysis, IP3, in the modulation of JH biosynthesis at specific developmental times and in glands showing specific levels of activity.
已研究了干扰1,4,5-肌醇三磷酸（IP3）/二酰甘油（DAG）途径的药物制剂对斑点折翅蠊咽侧体（CA）合成保幼激素（JH）的影响。在存在抑制性神经肽——咽侧体抑制素的情况下评估这些影响，以阐明抑制JH生物合成过程中的信号转导途径。用DAG激酶抑制剂处理CA以提高CA细胞内DAG的浓度，导致JH生物合成显著且呈剂量依赖性降低。用DAG激酶抑制剂和咽侧体抑制素同时处理腺体进一步增强了这种作用，表明DAG是咽侧体抑制素诱导的JH产生抑制作用中的一个中间产物。PKC的佛波酯激活剂PDBu或咽侧体抑制素对JH生物合成的抑制作用，通过用PKC抑制剂预孵育CA而被部分阻断。用钙动员药物毒胡萝卜素处理CA，导致高JH合成率的交配雌性腺体中JH生物合成显著增加。毒胡萝卜素还能够逆转高活性交配CA中咽侧体抑制素的作用。这表明磷酸肌醇水解的另一种产物IP3在特定发育阶段以及显示特定活性水平的腺体中参与JH生物合成的调节。
