Del Prete G, De Carli M, Almerigogna F, Daniel C K, D'Elios M M, Zancuoghi G, Vinante F, Pizzolo G, Romagnani S
Division of Clinical Immunology and Allergology, University of Florence, Italy.
FASEB J. 1995 Jan;9(1):81-6.
A large panel of human CD4+ T helper (Th) cell clones with established Th1, Th2, or Th0 profiles of cytokine secretion were examined for the expression of CD30, a member of the tumor necrosis factor receptor superfamily. Th1 clones expressed poor or no CD30 mRNA, and showed low or undetectable expression of both membrane and soluble CD30 (sCD30) protein, whereas Th2 clones showed both CD30 mRNA and membrane CD30 and released substantial amounts of sCD30. Th0 clones exhibited an intermediate pattern of CD30 expression and release. When T cells from the same donor were stimulated with three different antigens (purified protein derivative, PPD; Toxocara canis excretory/secretory antigen, TES; Lolium perenne group I, Lol p I), production of high concentrations of IFN-gamma, but not expression of CD30 or production of IL-4 and IL-5, were observed at any time after stimulation with PPD. In contrast, both CD30 expression and production of IL-4 and IL-5, but not of IFN-gamma, were concomitantly detectable in TES- and Lol p I-reactive T cells, suggesting a temporal relationship between CD30 expression and beginning of Th2-type cytokine production. Finally, CD4+CD30+ T cells specific for Lol p I and inducible to production of Th2-type cytokines were sorted out from the circulation of grass-sensitive patients in concomitance with the onset of allergic symptoms during the seasonal exposure to grass pollen. Thus, CD30 expression appears to be associated with the differentiation/activation pathway of human T cells producing Th2-type cytokines.
我们检测了一大组已确定细胞因子分泌具有Th1、Th2或Th0特征的人CD4 +辅助性T（Th）细胞克隆，以研究肿瘤坏死因子受体超家族成员CD30的表达情况。Th1克隆表达的CD30 mRNA很少或不表达，膜结合型和可溶性CD30（sCD30）蛋白的表达水平很低或无法检测到，而Th2克隆同时表达CD30 mRNA和膜结合型CD30，并释放大量的sCD30。Th0克隆表现出CD30表达和释放的中间模式。当用三种不同抗原（纯化蛋白衍生物，PPD；犬弓首蛔虫排泄/分泌抗原，TES；黑麦草I组，Lol p I）刺激来自同一供体的T细胞时，在PPD刺激后的任何时间都观察到高浓度干扰素-γ的产生，但未观察到CD30的表达或IL-4和IL-5的产生。相反，在对TES和Lol p I有反应的T细胞中可同时检测到CD30的表达以及IL-4和IL-5的产生，但未检测到干扰素-γ的产生，这表明CD30表达与Th2型细胞因子产生的开始之间存在时间关系。最后，从对草敏感患者的循环系统中筛选出对Lol p I特异且可诱导产生Th2型细胞因子的CD4 + CD30 + T细胞，此时正值季节性接触草花粉期间过敏症状发作之时。因此，CD30的表达似乎与人T细胞产生Th2型细胞因子的分化/激活途径有关。
