Inubushi T, Kakegawa H, Kishino Y, Katunuma N
Institute for Health Sciences, Tokushima Bunri University.
J Biochem. 1994 Aug;116(2):282-4. doi: 10.1093/oxfordjournals.jbchem.a124520.
We have established a new differential assay method for cathepsin L-type proteinases using specific inhibitors, E-64 for all cysteine proteinases, CA-074 for cathepsin B, and PLCPI for cathepsin L-type proteinases with Z-Phe-Arg-MCA as the substrate. The value of cathepsin B calculated by this method did not coincide with value assayed directly in terms of the hydrolysis of Z-Arg-Arg-MCA, a specific substrate for cathepsin B. The activities of cathepsin L-type proteinases, cathepsins B and J in rat liver and kidney were assayed at the same time using this new assay method as a representative example.
我们建立了一种新的组织蛋白酶L型蛋白酶的差异检测方法，该方法使用特异性抑制剂，即针对所有半胱氨酸蛋白酶的E-64、针对组织蛋白酶B的CA-074以及针对组织蛋白酶L型蛋白酶的PLCPI，并以Z-Phe-Arg-MCA作为底物。通过该方法计算得到的组织蛋白酶B的值，与使用组织蛋白酶B的特异性底物Z-Arg-Arg-MCA直接检测得到的值不一致。以这种新的检测方法为代表，同时检测了大鼠肝脏和肾脏中组织蛋白酶L型蛋白酶、组织蛋白酶B和组织蛋白酶J的活性。
