Khanna V K, Husain R, Seth P K
Industrial Toxicology Research Centre, Lucknow, India.
J Appl Toxicol. 1994 Sep-Oct;14(5):351-6. doi: 10.1002/jat.2550140506.
Neurotoxic effects of styrene on certain biochemical and behavioural indices were studied in young rats kept deficient in protein during the weaning period. Young rats fed a low-protein diet showed a significant decrease in the level of dopamine and an increase in frontocortical [3H]serotonin binding in comparison to the group of rats fed a normal protein diet. These rats also showed a significant increase in foot shock-induced aggressive behaviour, while no changes in amphetamine-induced locomotor activity, levels of norepinephrine and serotonin and binding of [3H]spiperone to striatal membrane were observed. On exposure to styrene, rats fed a normal protein diet showed a decrease in dopamine level and an increase in foot shock-induced aggressive behaviour only, with no significant change in other parameters, in comparison to the respective controls. It was, however, interesting to note that when rats fed a low-protein diet were exposed to styrene they showed a significant decrease in the levels of norepinephrine, dopamine and serotonin and an increase in the binding of [3H]spiperone and [3H]5-HT to striatal and frontocortical membranes, respectively. A significant increase in foot shock-induced aggressive behaviour and amphetamine-induced locomotor activity was also observed in this group of animals in comparison to those fed a low-protein diet. The biochemical and behavioural data indicate that protein deficiency makes young animals more vulnerable and it is an important predisposing factor in the neurobehavioural toxicity of styrene.
在断奶期蛋白质缺乏的幼鼠中，研究了苯乙烯对某些生化和行为指标的神经毒性作用。与喂食正常蛋白质饮食的大鼠组相比，喂食低蛋白饮食的幼鼠多巴胺水平显著降低，前额皮质[3H]5-羟色胺结合增加。这些大鼠足部电击诱发的攻击行为也显著增加，而苯丙胺诱发的运动活动、去甲肾上腺素和5-羟色胺水平以及[3H]螺哌隆与纹状体膜的结合均未观察到变化。与各自的对照组相比，暴露于苯乙烯时，喂食正常蛋白质饮食的大鼠仅多巴胺水平降低，足部电击诱发的攻击行为增加，其他参数无显著变化。然而，有趣的是，当喂食低蛋白饮食的大鼠暴露于苯乙烯时，它们的去甲肾上腺素、多巴胺和5-羟色胺水平显著降低，[3H]螺哌隆和[3H]5-羟色胺分别与纹状体膜和前额皮质膜的结合增加。与喂食低蛋白饮食的动物相比，这组动物中足部电击诱发的攻击行为和苯丙胺诱发的运动活动也显著增加。生化和行为数据表明，蛋白质缺乏使幼小动物更易受影响，并且是苯乙烯神经行为毒性的重要易感因素。
