Barradas M A, Stansby G, Hamilton G, Mikhailidis D P
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London, UK.
Int Angiol. 1994 Sep;13(3):202-7.
Centrifugation of citrated whole blood (cit-WB) for platelet rich plasma (PRP) preparation resulted in a significantly diminished platelet yield compared to when blood was collected in citrate + aspirin (acetylsalicylic acid; ASA) or EDTA anticoagulants in both apparently healthy young subjects and peripheral vascular disease (PVD) patients. The loss in platelets was significantly greater in PVD patients than in young apparently healthy subjects when using cit-WB. Median platelet volume (MePV) of PRP preparations was not different between PVD patients and apparently healthy subjects. The MePV of EDTA-PRP preparations was significantly bigger than cit-PRP and cit + ASA-PRP in both PVD patients and apparently healthy subjects. Citrate and citrate + ASA-PRP of patients was significantly more aggregable to adrenaline. In EDTA-PRP, adrenaline-induced aggregation did not occur. These findings indicate that platelet hyperactivity occurs in PVD even in the presence of ASA. Furthermore, studies involving the use of PRP may not be representative in PVD due to the significant loss of platelets during centrifugation. The methodology described here can be used by those who do not have facilities designed specifically for platelet research.
与在柠檬酸盐+阿司匹林（乙酰水杨酸；ASA）或乙二胺四乙酸（EDTA）抗凝剂中采集血液的情况相比，在明显健康的年轻受试者和外周血管疾病（PVD）患者中，用枸橼酸盐全血（cit-WB）离心制备富血小板血浆（PRP）时血小板产量显著降低。在使用cit-WB时，PVD患者血小板的损失明显大于明显健康的年轻受试者。PVD患者和明显健康受试者的PRP制剂的平均血小板体积（MePV）没有差异。在PVD患者和明显健康受试者中，EDTA-PRP制剂的MePV均显著大于cit-PRP和cit + ASA-PRP。患者的枸橼酸盐和枸橼酸盐+ ASA-PRP对肾上腺素的聚集性显著更高。在EDTA-PRP中，未发生肾上腺素诱导的聚集。这些发现表明，即使存在ASA，PVD中也会出现血小板活性过高。此外，由于离心过程中血小板大量损失，涉及使用PRP的研究在PVD中可能不具有代表性。没有专门用于血小板研究设施的人员可以使用此处描述的方法。
