通过免疫荧光法对猪生殖道中低分子量顶体蛋白酶-胰蛋白酶抑制剂进行定位。

Localization of low molecular weight acrosin-trypsin inhibitors in the boar genital tract by immunofluorescence.

作者信息

Schill W B, Schiessler H, Wendt V, Heimburger N, Fritz H

出版信息

Andrologia. 1976;8(1):29-36. doi: 10.1111/j.1439-0272.1976.tb01641.x.

DOI:10.1111/j.1439-0272.1976.tb01641.x

Abstract

Using the indirect immunofluorescent technique, the occurrence and distribution of the low molecular weight acid-stable acrosin inhibitors from boar seminal plasma (BSAI) in the boar genital tract was studied applying specific inhibitor-directed rabbit-immunoglobulins. The acrosin inhibitors are localized in the mucosa cells of the cauda epididymis, the vas deferens, the seminal vesicles, the urethra and distinct glandular units of the prostate. The wide distribution of the acrosin inhibitors within the boar genital tract indicates a protective function towards the destructive proteolytic potential of acrosin liberated e.g. during sperm aging from desintegrating spermatozoa.

摘要

利用间接免疫荧光技术，应用特异性抑制剂导向的兔免疫球蛋白，研究了公猪精液血浆中低分子量酸稳定顶体蛋白酶抑制剂（BSAI）在公猪生殖道中的发生和分布情况。顶体蛋白酶抑制剂定位于附睾尾、输精管、精囊、尿道以及前列腺的不同腺单位的黏膜细胞中。顶体蛋白酶抑制剂在公猪生殖道内的广泛分布表明，其对例如精子老化过程中从解体精子中释放的顶体蛋白酶的破坏性蛋白水解潜力具有保护作用。