Plotton N, Cavallo J D, Crenn Y, Meyran M
Laboratoire de Biologie Médicale, HIA Bégin, St-Mandé, France.
Pathol Biol (Paris). 1994 May;42(5):468-70.
E-test was evaluated by comparison with the reference agar dilution method on 15 enterococci and 15 staphylococci with variable resistance to glycopeptides and isolated from clinical samples: 15 enterococci with 3 glycopeptide-resistant (2 E. facalis and 1 E. faecium), 1 glycopeptide intermediate E. gallinarum and 11 glycopeptide-sensible E. faecalis; 15 staphylococci with 4 teicoplanine-resistant (1 S. aureus, 1 S. epidermidis and 2 S. hominis), 3 teicoplanin-intermediate (1 S. epidermidis, 2 S. haemolyticus) and 8 teicoplanin sensible strains (6 S. aureus, 1 S. epidermidis and 1 S. hominis). Repeatability (10 determinations by strain) and exactitude were tested for all strains. Our data shows very good results for repeatability and exactitude with the reference method except in the case of coagulase negative staphylococci and teicoplanin. Some technical problems appear in our experience: for staphylococci, a 48 h incubation is necessary to detect teicoplanin-intermediate strains but 24 h is sufficient for enterococci. E-test is expensive, require a strict methodology, but is a good method to detect glycopeptide resistance for staphylococci and enterococci except for coagulase negative staphylococci and teicoplanine.
通过与参考琼脂稀释法比较，对从临床样本中分离出的15株对糖肽具有不同耐药性的肠球菌和15株葡萄球菌进行E-test评估：15株肠球菌中，3株对糖肽耐药（2株粪肠球菌和1株屎肠球菌），1株鹑鸡肠球菌对糖肽中介，11株粪肠球菌对糖肽敏感；15株葡萄球菌中，4株对替考拉宁耐药（1株金黄色葡萄球菌、1株表皮葡萄球菌和2株人葡萄球菌），3株对替考拉宁中介（1株表皮葡萄球菌、2株溶血葡萄球菌），8株对替考拉宁敏感菌株（6株金黄色葡萄球菌、1株表皮葡萄球菌和1株人葡萄球菌）。对所有菌株测试了重复性（每个菌株测定10次）和准确性。我们的数据显示，除凝固酶阴性葡萄球菌和替考拉宁外，参考方法的重复性和准确性结果非常好。在我们的经验中出现了一些技术问题：对于葡萄球菌，检测对替考拉宁中介的菌株需要48小时培养，但对于肠球菌24小时就足够了。E-test成本高，需要严格的方法，但除凝固酶阴性葡萄球菌和替考拉宁外，是检测葡萄球菌和肠球菌糖肽耐药性的好方法。
