Monzon R B, Thammapalerd N, Kitikoon V, Temcharoen P, Sornmani S, Viyanant V
Department of Parasitology, UP College of Public Health, Manila, Philippines.
Southeast Asian J Trop Med Public Health. 1994 Mar;25(1):181-6.
Foot muscle tissue extracts from six lymnaeid species of the Indo-Pacific region [Lymnaea (Bullastra) cumingiana and L. (Radix) quadrasi from the Philippines, L. (R.) rubiginosa from Indonesia and Thailand, and L. (R.) viridis from Guam and Hong Kong] were subjected to horizontal starch gel isoenzyme electrophoresis and assayed for seven isoenzymes (AcP, AlP, CA, EST, LAP, CAT and GOT) to elucidate their taxonomic relationships. L. cumingiana exhibited banding patterns for EST, LAP and CAT uniquely different from the rest, thus supporting the hypothesis that it is a distinct species. Zymogram patterns for AlP, CA, EST and LAP attest to the close affinity between L. quadrasi and L. rubiginosa (Indonesia and Thailand). Minor differences suggest a closer relationship between the two geographical strains of L. rubiginosa than with L. quadrasi, lending support to the hypothesis that L. quadrasi is inseparable as a race or variety from the typical L. swinhoei Adams, which in turn is but a race of L. auricularia, which also encompasses L. rubiginosa. The two geographical strains of L. viridis from Guam and Hong Kong showed the greatest consistency with regards to similarity and congruence in banding patterns. Non-specific esterases (EST) were the most useful in distinguishing the six species from each other.
对来自印度 - 太平洋地区的六种椎实螺科物种的足部肌肉组织提取物进行了水平淀粉凝胶同工酶电泳分析，并检测了七种同工酶（酸性磷酸酶、碱性磷酸酶、碳酸酐酶、酯酶、亮氨酸氨基肽酶、过氧化氢酶和谷草转氨酶），以阐明它们的分类关系。累积椎实螺在酯酶、亮氨酸氨基肽酶和过氧化氢酶方面呈现出与其他物种独特不同的条带模式，从而支持了它是一个独特物种的假设。碱性磷酸酶、碳酸酐酶、酯酶和亮氨酸氨基肽酶的酶谱模式证明了菲律宾椎实螺和锈色椎实螺（印度尼西亚和泰国）之间的密切亲缘关系。细微差异表明锈色椎实螺的两个地理菌株之间的关系比与菲律宾椎实螺更密切，这支持了菲律宾椎实螺作为一个变种或品种与典型的斯氏椎实螺不可分割的假设，而斯氏椎实螺又是耳萝卜螺的一个变种，耳萝卜螺也包括锈色椎实螺。来自关岛和香港的绿色椎实螺的两个地理菌株在条带模式的相似性和一致性方面表现出最大的一致性。非特异性酯酶在区分这六个物种方面最有用。
