Mu M Y, Pao X, Jie T
Ear Nose and Throat Institute, Shanghai Medical University, People's Republic of China.
Acta Otolaryngol. 1994 Sep;114(5):516-9. doi: 10.3109/00016489409126096.
Scanning electronic microscopy (SEM) observation and GABA immunoreactivity of the tissue culture of 6 human embryonic organs of Corti are reported. The normal structural appearance of the hair cells and the facts that there were no bulging of cuticular plates, and cytoplasmic protrusion proved that the hair cells were healthy. After GABA immunostaining, radial bundles, innerspiral bundles, and the ending of inner and outer hair cell regions displayed typical GABA-positive reactivity. The results indicate that the human embryonic organ of Corti is well developed differentiated, and mature at 19 gestational weeks and can continue to grow and differentiate in vitro. The study offers an ideal and important biological model for further research into the human ear.
报道了对6个人类胚胎柯蒂氏器组织培养物的扫描电子显微镜（SEM）观察及γ-氨基丁酸（GABA）免疫反应性。毛细胞的正常结构外观以及角质板无隆起和细胞质突起的事实证明毛细胞是健康的。GABA免疫染色后，放射状束、内螺旋束以及内、外毛细胞区域的末端呈现典型的GABA阳性反应。结果表明，人类胚胎柯蒂氏器在妊娠19周时发育良好、分化成熟，且能在体外继续生长和分化。该研究为进一步研究人类耳朵提供了理想且重要的生物学模型。
