Sloane D L, Sigal E
Syntex Discovery Research, Palo Alto, California 94303.
Ann N Y Acad Sci. 1994 Nov 15;744:99-106. doi: 10.1111/j.1749-6632.1994.tb52727.x.
The lipoxygenases comprise a family of fatty acid dioxygenases that are involved in a variety of inflammatory conditions. Various approaches have been taken in order to understand the different regiospecificities of the different lipoxygenases. Here we have reviewed the current knowledge of the structural features of the substrate and of the enzyme that form the basis of the regiospecificity of 15-lipoxygenase. Earlier experiments on the structural features of the substrate were reviewed, as well as more recent results of site-directed mutagenesis studies. The structure of the soybean lipoxygenase isoform-1 was also briefly reviewed.
脂氧合酶是一类脂肪酸双加氧酶，参与多种炎症反应。为了解不同脂氧合酶的不同区域特异性，人们采取了多种方法。在此，我们综述了目前关于底物和酶的结构特征的知识，这些特征构成了15-脂氧合酶区域特异性的基础。回顾了早期关于底物结构特征的实验，以及定点诱变研究的最新结果。还简要综述了大豆脂氧合酶同工型-1的结构。
